Coding

Part:BBa_M11031:Design

Designed by: Erik Ostler   Group: UtahState BE5930 - S09 UtahState BE5930 - S10   (2009-04-15)

Endo-beta-1,4-gluconase [Ampullaria crossean]


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1118
    Illegal PstI site found at 1778
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1118
    Illegal PstI site found at 1778
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1118
    Illegal PstI site found at 1778
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1118
    Illegal PstI site found at 1778
    Illegal NgoMIV site found at 964
    Illegal AgeI site found at 374
    Illegal AgeI site found at 658
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Amino acid sequence was imported into DNA 2.0 where the most appropriate nucleic acid sequence was determined. Two stop codons were added to the end of the protein sequence and and OmpA secretion sequence was added to the beggining.

Source

Amino acid sequence was found in the NCBI database.

References

http://www.ncbi.nlm.nih.gov/nuccore/87133079?ordinalpos=1&itool=EntrezSystem2.PEntrez.Sequence.Sequence_ResultsPanel.Sequence_RVDocSum